Culminating Project Title
Date of Award
Culminating Project Type
College of Science and Engineering
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Toxoplasma gondii belongs to the phylum the Apicomplexa, a phylum that mainly consists of difficult to study intracellular protozoan parasites. The zoonosis caused by T. gondii is called toxoplasmosis. Even though most healthy individuals infected will be asymptomatic, a T. gondii infection can be lethal for individuals with a compromised immune system. Non-specific drugs exist to treat toxoplasmosis, but these are typically not able to eliminate T. gondii completely from the infected host and infection reoccurs. The proteins involved in T. gondii’s unique cell cycle are considered prime targets for the development of new anti-parasitic drugs. However, in order to target vital and unique T. gondii proteins these must first be identified and their interactions characterized. One such potential protein would be the putative T. gondii cell cycle protein TgCyc2. TgCyc2 has previously been found to interact with TgIMC1 and TgCDK1 in a yeast two-hybrid screen. It is hypothesized that TgCyc2 targets TgCDK1 to a specific suborganellar location by binding to the inner membrane protein TgIMC1. Here an initial attempt to elucidate this mechanism via a yeast two-hybrid test is described.
Hinz, Sandra M., "Mapping the Interaction Between Two Toxoplasma gondii Cell Cycle Proteins." (2017). Culminating Projects in Biology. 24.