Event Title

The Effect of Sodium Bicarbonate Treatment on the Inflammatory Process in Type 1 Diabetic Mice

Presentation Type

Poster

Start Date

23-4-2019 12:00 AM

End Date

23-4-2019 12:00 AM

Description

Award for Runner Up Undergraduate Poster.

Abstract

Around 1.25 million Americans live with Type 1 Diabetes (T1D), an autoimmune disease that develops when insulin-producing __ cells in the pancreas are destroyed by an inflammatory process led by T cells. The absence of insulin induces hyperglycemia (high blood glucose levels) accompanied by numerous complications such as retinopathy, kidney and heart disease, nerve damage, and slow wound healing. There are several types of T cells; some directly kill __ cells, such as T-cytotoxic (Tc), or indirectly, such as T-helper (Th), while others, like regulatory T cells (Treg) actually protect them. A recent study showed that sodium bicarbonate (NaHCO3) induces anti-inflammatory pathway in the spleen by affecting a particular type of immune cells, macrophages. Therefore, it is hypothesized that NaHCO3 treatment will affect other inflammatory cells, such as Tc and Th populations. In order to test this hypothesis, T1D was induced in two groups of 7-week old male C57Bl/6 mice using streptozotocin (STZ) injections. Control group drank normal water whereas treatment group drank 200 mM NaHCO3-enriched water. On Day 30 of the experiment, mice were euthanized, and their spleens obtained for analysis of cell counts, viability, T cell proliferation, and differentiation / quantification of T cell subsets by flow cytometry. Whereas there were no signficant differences in splenic lymphocyte counts and viability between control and treatment mice, a trend of decreasing Th and Tc populations was observed in NaHCO3-treated mice. These preliminary results suggest that NaHCO3 might exhibit anti-inflammatory properties in its action on T cells, while not inducing toxic effects on treated mice.

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Apr 23rd, 12:00 AM Apr 23rd, 12:00 AM

The Effect of Sodium Bicarbonate Treatment on the Inflammatory Process in Type 1 Diabetic Mice

Award for Runner Up Undergraduate Poster.

Abstract

Around 1.25 million Americans live with Type 1 Diabetes (T1D), an autoimmune disease that develops when insulin-producing __ cells in the pancreas are destroyed by an inflammatory process led by T cells. The absence of insulin induces hyperglycemia (high blood glucose levels) accompanied by numerous complications such as retinopathy, kidney and heart disease, nerve damage, and slow wound healing. There are several types of T cells; some directly kill __ cells, such as T-cytotoxic (Tc), or indirectly, such as T-helper (Th), while others, like regulatory T cells (Treg) actually protect them. A recent study showed that sodium bicarbonate (NaHCO3) induces anti-inflammatory pathway in the spleen by affecting a particular type of immune cells, macrophages. Therefore, it is hypothesized that NaHCO3 treatment will affect other inflammatory cells, such as Tc and Th populations. In order to test this hypothesis, T1D was induced in two groups of 7-week old male C57Bl/6 mice using streptozotocin (STZ) injections. Control group drank normal water whereas treatment group drank 200 mM NaHCO3-enriched water. On Day 30 of the experiment, mice were euthanized, and their spleens obtained for analysis of cell counts, viability, T cell proliferation, and differentiation / quantification of T cell subsets by flow cytometry. Whereas there were no signficant differences in splenic lymphocyte counts and viability between control and treatment mice, a trend of decreasing Th and Tc populations was observed in NaHCO3-treated mice. These preliminary results suggest that NaHCO3 might exhibit anti-inflammatory properties in its action on T cells, while not inducing toxic effects on treated mice.