Culminating Project Title
Date of Award
Culminating Project Type
Biological Sciences - Cell and Molecular: M.S.
College of Science and Engineering
Creative Commons License
This work is licensed under a Creative Commons Attribution-Noncommercial-No Derivative Works 4.0 License.
Keywords and Subject Headings
Toxoplasma, Apicomplexa, Cell Cycle, Regulation, Proteins
Toxoplasma gondii is an Apicomplexan obligate intracellular protozoan parasite, which is able to infect virtually all warm-blooded animals. According to the Centers for Disease Control approximately 60 million people in the U.S. are currently infected with T. gondii. Treatments for toxoplasmosis are limited and generally consist of a combination of pharmaceuticals. Based on the prevalence and limited options for treatment new drug targets should be explored. Cell cycle proteins often present themselves as good drug targets. TgCDK1 and TgCYC2 are cell cycle proteins. TgCDK1 is a cyclin dependent kinase and TgCYC2 is thought to be a cyclin protein. TgCDK1 and TgCYC2 are associated with the G2 to M phase regulation of the cell cycle. Inhibition of the cell cycle would prevent the cell from proliferating ultimately eliminating T. gondii from the host. TgCDK1 and TgCYC2 proteins must first be purified and a kinase assay has to be run in order to determine the function of these two proteins. Experimental approaches to the purification and characterization of these proteins refines the search for drug targets.
Barnes, Blake Heim, "Expression and Purification of Toxoplasma gondii Cell Cycle Regulation Proteins" (2016). Culminating Projects in Biology. 13.