Lecture 28: Protein folding and unfolding

Nathan S. Winter, St. Cloud State University

NOTE: For closed captioning video, click on the "Link to Full Text" button to the upper right.


This lecture in Biochemistry addressees denaturation and the folding problem. Some of the means to denature a protein are mentioned, including temperature, pH, hydrophobic solvents, detergents and chaotropic reagents (urea and the guanidinium ion). How proteins (which are synthesized like beads on a string) fold to functional tertiary structures are examined with particular attention spent on ribonuclease A.. The steps and energetics of the folding process are discussed.