Culminating Project Title
Date of Award
Culminating Project Type
Biological Sciences - Cell and Molecular: M.S.
College of Science and Engineering
Creative Commons License
This work is licensed under a Creative Commons Attribution-Noncommercial-No Derivative Works 4.0 License.
Keywords and Subject Headings
type 1 diabetes, garcinia kola, T cells, autoimmune disease, C57BL/6J mice
Type 1 diabetes (T1D) is a non-preventable autoimmune disease where autoreactive T cells orchestrate the destruction of insulin-producing pancreatic beta cells. Individuals with T1D are committed to a life-long dependency on insulin, which it and T1D associated care is expensive, and in many regions around the world is unattainable. Thus, there is an imperative to find an accessible and efficient alternative. The seeds of Garcinia kola are commonly used for cultural and medicinal purposes in West Africa. Previous studies have shown that Garcinia kola seed extract (GKE) has hypoglycemic and anti-inflammatory effects which suit is as a candidate for preventing disease development. It was hypothesized that treatment with GKE would prevent T1D development through its anti-inflammatory action in an experimental mouse model. Male seven-week-old C57BL/6J mice were treated with aqueous or ethanol GKE (100 mg/kg) in their drinking water for five weeks and were chemically induced with T1D at eight weeks of age. Biweekly body weight and glycemia measurements were performed from nine to 13 weeks of age. Mice were euthanized at days 11 and 30 after the initial STZ injection to evaluate GKE’s effects on T cells. Treatment with GKE had no effect on glycemia, diabetes incidence or prevalence. However, the immunomodulatory activity of GKE displayed a shift to a pro-inflammatory response, suggesting potential therapeutic benefits for other diseases. This study did not confirm the anti-diabetic potential of GKE, however, it did highlight the need for more consistent research on herbal compounds.
Rogan, Shana, "Garcinia kola Extract Potentiates a Pro-inflammatory Response While Not Affecting Type 1 Diabetes Incidence in an Experimental Mouse Model" (2018). Culminating Projects in Biology. 38.