The Repository @ St. Cloud State

Open Access Knowledge and Scholarship

Date of Award


Culminating Project Type


Degree Name

Biological Sciences - Cell and Molecular: M.S.




College of Science and Engineering

First Advisor

Marina Cetkovic-Cvrlje

Second Advisor

Latha Ramakrishnan

Third Advisor

Timothy Schuh

Creative Commons License

Creative Commons License
This work is licensed under a Creative Commons Attribution-Noncommercial-Share Alike 4.0 License.

Keywords and Subject Headings

Immunotoxicology, environment, children's health, cytokines, polychlorinated biphenyls


Type 1 diabetes {Tl D) is an autoimmune disorder characterized by the Tcell- mediated destruction of insulin-producing P-cells in the pancreatic islets of Langerhans. The steady increase in prevalence and incidence of Tl D across the globe suggests disease onset may be contributed by various environmental factors besides genetics, such as persistent organic pollutants (POP). Given that polychlorinated biphenyl (PCB)-153 is a highly abundant POP in both the environment and mammalian tissues, there is reason to believe the compound may be an environmental factor influencing disease susceptibility and onset. Non-obese diabetic (NOD) mice, the best experimental model for studying TID, were exposed to intraperitoneal injections of PCB-153 in a 10-day acute (50mg/Kg or 0.5mg/Kg) or 16-week chronic (12.5mg/Kg or 0.125mg/Kg) fashion. Analysis of various immune parameters, including T-cell types and subtypes, T-cell proliferative responses, as well as their cytokine secretions, revealed that both acute and chronic exposure to PCB-153 caused significant immunosuppression in all PCB-153- exposed mice. Based on the significant decreases in c04+ T-helper cells and reduced secretion of interleukin {IL)-2 it is plausible to believe that T-helper 1 {TH1) cells are the most susceptible cell population to PCB-153 exposure. This is further supported by the decrease in TlD incidence observed in mice chronically exposed to either dose of PCB-153. Overall, this study not only reveals for the first time the protective effects of PCB-153 exposure on TlD, but brings the awareness about PCB-153's "antidiabetogenic" immunosuppressive effects in the context of influencing the action of other co-pollutants, as PCB-153 might mask other pollutants' effects on TIO development.

Included in

Biology Commons



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