The Repository @ St. Cloud State

Open Access Knowledge and Scholarship

Date of Award


Culminating Project Type




Degree Name

Biological Sciences - Ecology and Natural Resources: M.S.




College of Science and Engineering

First Advisor

Matthew Julius

Second Advisor

Angela McDonnell

Third Advisor

Tracy Ore

Creative Commons License

Creative Commons License
This work is licensed under a Creative Commons Attribution-Noncommercial-No Derivative Works 4.0 License.

Keywords and Subject Headings

Carbon Dioxide, Agriculture


Metabolism control of plants has contributed to advancements in biopharming and horticulture over the last few decades. The last element that has yet to be controlled when talking about plants is carbon dioxide (CO2). Being able to control this element would lead to increased production for enclosed agriculture and industrial uses. The purpose of this study is to provide a carbon neutral method of controlling the metabolism of plants utilizing CO2 to optimize production yields. It began with developing the methodology to provide optimal CO2 coverage and saturation to the leaves, bypassing the stomata, the plants method of obtaining carbon. Experimenting on mister heads and mister head spacing for optimal leaf surface area coverage, creating a biofilm of concentrated carbon was the next step in controlling CO2 delivery. Verification of carbon delivery and increased photosynthesis and carbon fixation was done through the quantification of introduced agrobacterium to the subject plant, Solanum Lycopersicum (Better Boy). in treatment and control groups. This concluded that metabolism control occurred utilizing this novel carbon delivery method.


I am overwhelmingly thankful for those who helped me throughout the process of completing my master’s thesis. I would first like to thank my advisor, Matthew Julius, who continuously supported me and provided a wealth of knowledge, guidance, support, and experience throughout the research and writing process. I am so grateful for all of his time and efforts. I would next like to thank my committee members, Angela McDonnell and Tracy Ore, who have generously given their time, support and expertise throughout my thesis writing and defense. I am thankful for my classmates Mason Olinger, Krissandra McLaughlin, and Aurora Lundberg who helped me succeed offering endless help and support. I am grateful for my family and friends who have encouraged me throughout my studies and provided endless support. Finally, I would like to thank St. Cloud State University for providing me with the opportunity to be a graduate assistant and receive my master’s degree.

Available for download on Thursday, January 02, 2025