Presentation Type
Poster
Location
Atwood Memorial Student Union
Start Date
11-4-2018 12:00 AM
End Date
11-4-2018 12:00 AM
Description
Award for "Best Our Husky Compact Reflection: Think Creatively and Critically".
Award for "Best Poster Presentation".
Abstract
Type 1 diabetes (T1D) is an autoimmune disease that results from the attack of auto-reactive lymphocytes (T-cells) on pancreatic beta cells causing hyperglycemia. Garcinia kola (GK), an African herb, is believed to have anti-inflammatory abilities. We hypothesized that GK extract (GKE) will reduce the activity of pathogenic T-cells, thus having a potential for prevention of T1D in a T1D mouse model. C5BL/6 mice were treated daily by GKE (100 mg/kg) added to their drinking water from 7 to 13 weeks of age, and diabetes was induced by the administration of streptozotocin. The GKE effects were tested on T-cell composition (by using flow cytometry) and T-cell function (by performing T-cell proliferation assays) in the treated and control mice. At the completion of the experiment, both control and treatment mice were euthanized, single cell suspension was prepared from their spleens, and cell counts and visibly obtained T-cell proliferation was tested by culturing splenocytes with the addition of T-cell-specific motogen concanavalin A, while identification of T-cells and their respective subpopulations was performed by exposing splenic cells to fluorochrome-labeled antibodies that detect specific T-cell markers, and their quantification was completed by laser-based flow cytometry. Our results showed that GKE treatment inhibited the proliferative capability of T-cells and decreased the percentage fo T-cells, as well as their subpopulations. In conclusion, our results show that GKE has a potential to act as an immunosuppressive T-cell agent that might positively affect T1D development.
Included in
Biology Commons, Endocrinology, Diabetes, and Metabolism Commons, Nutritional and Metabolic Diseases Commons
An African Herbal Extract Affects Immune Response in Type 1 Diabetic Mouse Model
Atwood Memorial Student Union
Award for "Best Our Husky Compact Reflection: Think Creatively and Critically".
Award for "Best Poster Presentation".
Abstract
Type 1 diabetes (T1D) is an autoimmune disease that results from the attack of auto-reactive lymphocytes (T-cells) on pancreatic beta cells causing hyperglycemia. Garcinia kola (GK), an African herb, is believed to have anti-inflammatory abilities. We hypothesized that GK extract (GKE) will reduce the activity of pathogenic T-cells, thus having a potential for prevention of T1D in a T1D mouse model. C5BL/6 mice were treated daily by GKE (100 mg/kg) added to their drinking water from 7 to 13 weeks of age, and diabetes was induced by the administration of streptozotocin. The GKE effects were tested on T-cell composition (by using flow cytometry) and T-cell function (by performing T-cell proliferation assays) in the treated and control mice. At the completion of the experiment, both control and treatment mice were euthanized, single cell suspension was prepared from their spleens, and cell counts and visibly obtained T-cell proliferation was tested by culturing splenocytes with the addition of T-cell-specific motogen concanavalin A, while identification of T-cells and their respective subpopulations was performed by exposing splenic cells to fluorochrome-labeled antibodies that detect specific T-cell markers, and their quantification was completed by laser-based flow cytometry. Our results showed that GKE treatment inhibited the proliferative capability of T-cells and decreased the percentage fo T-cells, as well as their subpopulations. In conclusion, our results show that GKE has a potential to act as an immunosuppressive T-cell agent that might positively affect T1D development.
