Presentation Type
Poster
Location
St. Cloud
Start Date
19-4-2022 9:00 AM
End Date
19-4-2022 9:30 AM
Description
It is known that the Westernized diet in the United States contains excess sugar, and paired with minimal exercise, can lead to health impairments like type 2 diabetes. Whereas a correlation with minimal exercise and increased sugar intake has been found with type 2 diabetes, yet this influence is unknown for type 1 diabetes. Type 1 diabetes, which has a prominent autoimmune background, may be affected by excessive consumption of soft drinks. Thus, a model of a spontaneous type 1 diabetic, non-obese-diabetic (NOD) mouse, will be used to test the effects of Coca-Cola on the development of the disease. We hypothesize that Coca-Cola will exacerbate type 1 diabetes onset and glycemia levels in female NOD mice. Young pre-diabetic 5–7-week- old female NOD mice will be randomly put into the groups that will either drink Coca-Cola (n=15), sugar-water (n=15), or autoclaved water (n=31). The mice will be taken off Coca-Cola or sugar-water after six weeks of treatment and will continue to drink autoclaved water until 24 weeks of age, when about 60-80% of the control mice should become diabetic, based on our animal colony’s historical data. The glucose levels and body weights will be recorded weekly. At either 24 weeks of age or when hyperglycemia is present for two consecutive measurements, the mice will be sacrificed. Data will be analyzed for a statistical difference in diabetes incidence, glycemic levels, and body weight gains between the treatment groups.
Included in
Does Coca-Cola Consumption Potentiate Diabetes Development in NOD Mice?
St. Cloud
It is known that the Westernized diet in the United States contains excess sugar, and paired with minimal exercise, can lead to health impairments like type 2 diabetes. Whereas a correlation with minimal exercise and increased sugar intake has been found with type 2 diabetes, yet this influence is unknown for type 1 diabetes. Type 1 diabetes, which has a prominent autoimmune background, may be affected by excessive consumption of soft drinks. Thus, a model of a spontaneous type 1 diabetic, non-obese-diabetic (NOD) mouse, will be used to test the effects of Coca-Cola on the development of the disease. We hypothesize that Coca-Cola will exacerbate type 1 diabetes onset and glycemia levels in female NOD mice. Young pre-diabetic 5–7-week- old female NOD mice will be randomly put into the groups that will either drink Coca-Cola (n=15), sugar-water (n=15), or autoclaved water (n=31). The mice will be taken off Coca-Cola or sugar-water after six weeks of treatment and will continue to drink autoclaved water until 24 weeks of age, when about 60-80% of the control mice should become diabetic, based on our animal colony’s historical data. The glucose levels and body weights will be recorded weekly. At either 24 weeks of age or when hyperglycemia is present for two consecutive measurements, the mice will be sacrificed. Data will be analyzed for a statistical difference in diabetes incidence, glycemic levels, and body weight gains between the treatment groups.
Comments
Faculty mentor: Marina Cetkovic-Cvrlje, Department of Biology
Runner-Up Undergraduate Poster Presentation
Keywords: Coca-Cola, autoimmunity, type 1 diabetes, NOD mice, sugar